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2007
OMIG, Abstract 12
OMIG
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The Ocular Distribution and Kinetics of Moxifloxacin Following Prophylactic Dosing Regimens and an Intracameral Injection in Rabbits.
G.R. Owen, L.F. Bernal-Perez, A.C. Brooks
Alcon Research, Ltd., Fort Worth, TX
Purpose: To determine the concentration of moxifloxacin in ocular tissues following two prophylactic topical dosing regimens, and after an intracameral injection in a rabbit model that mimics the human eye.
Methods: Rabbits were dosed topically with moxifloxacin 0.5% (Vigamox ®) by two different regimens: A) four doses every 15 minutes for one hour prior to collection, or B) a single dose one hour before collection. The rabbit model that was used mimicked the human eye by manual blinking at the rate of 4 blinks/min and adding supplementary tears at the rate of 2 μL/min. The third group of rabbits (C) were given an intracameral injection of moxifloxacin 0.5% (50 μL) into the aqueous humor.
The concentration of moxifloxacin (μg/mL or μg/g) was determined by HPLC.
Results:
Regimen |
A |
B |
C |
Time |
At Collection |
Initial |
4 Hrs |
6 Hrs |
12 Hrs |
Aqueous Humor |
7 |
1.4 |
710 |
6.0 |
0.5 |
0.2 |
Cornea |
32 |
6.9 |
- |
17.2 |
1.3 |
0.4 |
Conjunctiva |
8 |
0.4 |
- |
0.4 |
<0.05 |
<0.05 |
Iris-Ciliary Body |
6 |
0.9 |
- |
2.7 |
0.3 |
0.2 |
Conclusions: The moxifloxacin MIC for a typical fluoroquinolone resistant isolate of Staphylococcus aureus and S. epidermidis is 2.0 μg/mL (Surv. Ophthal. 2005; 50:S16-31). Topical dosing by regimen A resulted in an aqueous humor concentration more than 3x this MIC at the time of collection. This is more than 100x the MIC (0.05 μg/mL) of fluoroquinolone sensitive staphylococci. An intracameral injection of Vigamox ® (C) maintained this same concentration (6-7 μg/mL) through 4 hours post surgery.
Disclosure code: E
OMIG
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